2R5F
Putative sugar-binding domain of transcriptional regulator DeoR from Pseudomonas syringae pv. tomato
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97907, 0.97921 |
| Spacegroup name | P 43 |
| Unit cell lengths | 42.112, 42.112, 144.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.400 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.477 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.081 | 0.434 |
| Number of reflections | 13180 | |
| <I/σ(I)> | 10.6 | 2.65 |
| Completeness [%] | 89.5 | 72.7 |
| Redundancy | 7.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M Bis-Tris pH 6.5, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
