2R55
Human StAR-related lipid transfer protein 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03992 |
| Spacegroup name | P 65 |
| Unit cell lengths | 62.870, 62.870, 214.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 2.500 |
| R-factor | 0.23633 |
| Rwork | 0.232 |
| R-free | 0.27597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jss |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 | 0.671 |
| Number of reflections | 16487 | |
| <I/σ(I)> | 23.25 | 5.94 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 22.4 | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10% PEG 6000, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
