2R49
Mutational and Structural Studies of E85I Reveal the Flexible Loops of Fibrobacter succinogenes 1,3-1,4-beta-D-GlucanaseGlucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.590, 69.590, 97.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.880 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zm1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.242 | |
| Number of reflections | 14319 | |
| <I/σ(I)> | 14.2 | 8.14 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 298 | 30% PEG6000, 0.1M CsCl, 0.1M MES (pH5.6), pH6.5, LIQUID DIFFUSION, temperature 298K |
