2R3M
Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.240, 70.870, 72.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.700 - 1.700 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.21600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | throughout |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.700 | 5.810 | 1.700 |
Rmerge | 0.037 | 0.020 | 0.584 |
Number of reflections | 29655 | ||
<I/σ(I)> | 22.5 | ||
Completeness [%] | 96.4 | 90.3 | 94.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 298 | 50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% PEG 4000, 4% Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K |