2R3H

Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-01
Detector	MARRESEARCH
Wavelength(s)	0.9510
Spacegroup name	P 21 21 21
Unit cell lengths	53.090, 70.680, 72.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 1.500
R-factor	0.196
Rwork	0.195
R-free	0.21900
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.500	5.130	1.500
Rmerge	0.047	0.032	0.340
Number of reflections	43774
<I/σ(I)>	21.7
Completeness [%]	97.8	94.4	55.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% v/v PEG 4000, 4% v/v Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K