2R3F

Crystal Structure of Cyclin-Dependent Kinase 2 with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.160, 71.450, 72.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.500
R-factor	0.205
Rwork	0.204
R-free	0.23600
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.500	5.130	1.500
Rmerge	0.060	0.046	0.183
Number of reflections	39402
<I/σ(I)>	23.3
Completeness [%]	88.2	67.9	49.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	50 mM Na-HEPES pH 7.4, 50 mM Ammonium Acetate, 8% PEG 4000, 4% Glycerol, 1 mM TCEP, vapor diffusion, temperature 298K