2R30
Crystal Structure of human GITRL mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 72.210, 72.210, 52.937 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.800 - 3.200 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.141 | 0.560 |
| Number of reflections | 2580 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 96.7 | 97 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 25% PEG 3350, 0.1M Sodium Acetate pH 4.5, Vapor diffusion, Sitting drop, temperature 298K |
