2R2Z
The crystal structure of a hemolysin domain from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 65 |
| Unit cell lengths | 39.494, 39.494, 101.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.750 - 1.200 |
| R-factor | 0.15531 |
| Rwork | 0.154 |
| R-free | 0.17082 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.279 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.750 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.117 | 0.318 |
| Number of reflections | 27135 | |
| <I/σ(I)> | 50.6 | 4.2 |
| Completeness [%] | 97.2 | 82.6 |
| Redundancy | 8.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 15% Ethanol, 0.1M MES, 0.2M Zn(OAC)2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
