2R2V
Sequence Determinants of the Topology of the Lac Repressor Tetrameric Coiled Coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 43 |
| Unit cell lengths | 51.696, 51.696, 99.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.710 - 1.900 |
| R-factor | 0.19347 |
| Rwork | 0.191 |
| R-free | 0.24028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nrn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.088 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.700 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.484 |
| Number of reflections | 20545 | |
| <I/σ(I)> | 13.2 | 3.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 0.1M sodium citrate, 5% Jeffamine M-600, 0.05M zinc acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
