2R2T
d(ATTTAGTTAACTAAAT) complexed with MMLV RT catalytic fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.068828 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.641, 145.801, 46.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.224 |
| R-free | 0.26400 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.191 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.060 | 0.034 | 0.298 |
| Number of reflections | 25162 | ||
| <I/σ(I)> | 15 | ||
| Completeness [%] | 96.4 | 94.2 | 81.3 |
| Redundancy | 4.3 | 4.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 4000, MgCl2, pH 6.5, vapor diffusion, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 2 | PEG 4000 | ||
| 4 | 1 | 2 | MgCl2 |
