2R2P

Kinase domain of human ephrin type-A receptor 5 (EphA5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Detector technology	CCD
Collection date	2007-06-11
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 65 2 2
Unit cell lengths	80.085, 80.085, 169.237
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.770 - 2.400
R-factor	0.20291
Rwork	0.199
R-free	0.28338
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsf
RMSD bond length	0.012
RMSD bond angle	1.323
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	13160
<I/σ(I)>	14.9	1.8
Completeness [%]	99.0	100
Redundancy	5.4	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	298	2M Ammonium sulfate, 2% PEG 400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K