2R2J
crystal structure of human ERp44
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.501, 83.501, 123.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.360 - 2.600 |
| R-factor | 0.21911 |
| Rwork | 0.217 |
| R-free | 0.26302 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.946 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.360 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.054 | 0.394 |
| Number of reflections | 15728 | |
| <I/σ(I)> | 26.7 | 2.5 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 6.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | disodium succinate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
