2R2E
Crystal structure of S25-2 Fab in complex with Kdo analogues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.110, 81.170, 132.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.400 |
| R-factor | 0.24673 |
| Rwork | 0.243 |
| R-free | 0.30618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.456 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.870 | 19.870 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.140 | 2.400 |
| Rmerge | 0.059 | 0.042 | 0.213 |
| Number of reflections | 17962 | ||
| <I/σ(I)> | 12.3 | 24.5 | 4 |
| Completeness [%] | 95.5 | 87.9 | 98.2 |
| Redundancy | 4.16 | 4.27 | 3.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | PEG 4000, ethelyene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
