2R2A
Crystal structure of N-terminal domain of zonular occludens toxin from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.976, 48.258, 95.894 |
| Unit cell angles | 90.00, 134.31, 90.00 |
Refinement procedure
| Resolution | 34.400 - 1.820 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.21440 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.400 | 1.900 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.101 | 0.543 |
| Number of reflections | 38209 | |
| <I/σ(I)> | 28.7 | 2.12 |
| Completeness [%] | 96.4 | 74.9 |
| Redundancy | 6.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.2M Ammonium sulfate, 0.1M Sodium citrate, 20% PEG 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
