2R28
The complex Structure of Calmodulin Bound to a Calcineurin Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-02 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 0.91240 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.700, 43.200, 70.900 |
Unit cell angles | 90.00, 109.90, 90.00 |
Refinement procedure
Resolution | 66.670 - 1.860 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f2o |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.670 | 1.910 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.069 | 0.759 |
Number of reflections | 28898 | |
<I/σ(I)> | 9 | 8.1 |
Completeness [%] | 98.1 | 98.1 |
Redundancy | 4.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 20% PEG 3350, 0.2M ammonium phosphate, 0.1M citrite acid , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |