2R25
Complex of YPD1 and SLN1-R1 with bound Mg2+ and BeF3-
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.013, 75.544, 98.392 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.750 - 1.700 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qsp |
RMSD bond length | 0.032 |
RMSD bond angle | 2.565 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.048 | 0.390 |
Number of reflections | 51329 | |
<I/σ(I)> | 28.8 | |
Completeness [%] | 97.9 | 99.6 |
Redundancy | 6.1 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 295 | 2.2 M ammonium sulfate, 50 mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 5.60 |