2R1Z
Crystal Structure of the BARD1 BRCT Repeat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.821, 75.550, 117.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 2.100 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.207 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.032 | 0.021 | 0.437 |
| Number of reflections | 28825 | ||
| <I/σ(I)> | 27.6 | ||
| Completeness [%] | 94.9 | 98.9 | 70 |
| Redundancy | 3.5 | 3.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | pSDDE peptide, 20% PEG 3350, 0.2 M ammonium chloride, 100 mM NaCl, 5 mM tris-HCl, 1 mM DTT, pH 7.5, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
