2R1S
Structure of the RNA brominated tridecamer r(GCGUU-5BUGAAACGC) at 1.4 A (Br1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.919487 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.380, 25.670, 29.920 |
| Unit cell angles | 98.64, 105.43, 100.67 |
Refinement procedure
| Resolution | 14.100 - 1.400 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.18500 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.100 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.035 | 0.113 |
| Number of reflections | 12174 | |
| <I/σ(I)> | 19.4 | 8.8 |
| Completeness [%] | 91.0 | 67.2 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 30% MPD, 100mM NaCl, 50 mM Tris, 3mM MnCl2, 2mM MgCl2, 0.5 mM RNA, pH 7.0, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | MgCl2 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 1 | MgCl2 | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | Tris | ||
| 9 | 1 | 2 | MnCl2 |
