2R1I
Crystal structure of putative acetyltransferase (YP_831484.1) from Arthrobacter sp. FB24 at 1.65 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97929, 0.97905 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.100, 40.260, 81.270 |
| Unit cell angles | 90.00, 123.39, 90.00 |
Refinement procedure
| Resolution | 27.746 - 1.650 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20600 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.615 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.746 | 27.746 | 1.710 |
| High resolution limit [Å] | 1.650 | 2.820 | 1.650 |
| Rmerge | 0.027 | 0.019 | 0.383 |
| Number of reflections | 46392 | ||
| <I/σ(I)> | 13.07 | 28.1 | 1.9 |
| Completeness [%] | 89.7 | 93.8 | 78.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | NANODROP, 15.0% Glycerol, 8.5% Isopropanol, 17.0% PEG 4000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
