2R18
Structural insights into the multifunctional protein VP3 of Birnaviruses
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0397, 1.04, 1.037 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.316, 68.316, 71.295 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.135 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.930 | 2.300 |
| Rmerge | 0.077 | 0.046 | 0.305 |
| Number of reflections | 7401 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 92.7 | 99.1 | 63.5 |
| Redundancy | 6.2 | 7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 2.5M NaCl 0.1M Sodium Acetate pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
