2R18

Structural insights into the multifunctional protein VP3 of Birnaviruses

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0397, 1.04, 1.037
Spacegroup name	P 41 21 2
Unit cell lengths	68.316, 68.316, 71.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.224
Rwork	0.222
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.135
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.380
High resolution limit [Å]	2.300	4.930	2.300
Rmerge	0.077	0.046	0.305
Number of reflections	7401
<I/σ(I)>	11.8
Completeness [%]	92.7	99.1	63.5
Redundancy	6.2	7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	2.5M NaCl 0.1M Sodium Acetate pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K