2R17
Functional architecture of the retromer cargo-recognition complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.250, 128.471, 140.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.095 | 0.071 | 0.332 |
| Number of reflections | 29916 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 98.8 | 99.8 | 91.7 |
| Redundancy | 13.5 | 13.4 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8 | 291 | 20% PEG 3350, 1M NaCl,50mM Tris, pH 8.0, hanging drop, temperature 291K |
