2R10

Structure of an acetylated Rsc4 tandem bromodomain Histone Chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-01
Detector	ADSC QUANTUM 4
Spacegroup name	C 2 2 21
Unit cell lengths	86.089, 90.958, 263.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.199
Rwork	0.196
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.495
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.078	0.494
Number of reflections	49197
<I/σ(I)>	17.2
Completeness [%]	95.3	83.9
Redundancy	3.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	298	0.1M Tris, 0.2M lithium sulfate, 28% PEG 3000, pH 8.5, VAPOR DIFFUSION, temperature 298K, pH 8.50