2R0W
PFA2 FAB complexed with Abeta1-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.793, 43.327, 58.456 |
| Unit cell angles | 92.51, 94.96, 90.55 |
Refinement procedure
| Resolution | 42.640 - 2.503 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.573 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.640 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.071 | 0.031 | 0.473 |
| Number of reflections | 13814 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 96.0 | 98.2 | 89.8 |
| Redundancy | 1.9 | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.3 | 295 | 25% PEG-MME 5000, 0.1 M OAc, pH 5.3, VAPOR DIFFUSION, temperature 295K |
