2R0Q
Crystal structure of a serine recombinase- DNA regulatory complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91977, 0.91988 |
| Spacegroup name | P 65 |
| Unit cell lengths | 132.500, 132.500, 315.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Rwork | 0.267 |
| R-free | 0.29100 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.340 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.136 | |
| Number of reflections | 55122 | |
| <I/σ(I)> | 12.44 | 2.87 |
| Completeness [%] | 96.9 | 95.6 |
| Redundancy | 7.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | drops containing 25 mM TRIS and 100 mM ammonium sulfate were mixed 1:1 with the sin-dna complex solution, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | drops containing 25 mM TRIS and 100 mM ammonium sulfate were mixed 1:1 with the sin-dna complex solution, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TRIS | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | TRIS | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | H2O |
