2QZE
Monoclinic Mimivirus Capping Enzyme Triphosphatase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2007-02-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.601 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.184, 92.401, 72.809 |
| Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.900 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qy2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 10635 | |
| <I/σ(I)> | 14.91 | 2.32 |
| Completeness [%] | 94.4 | 84.6 |
| Redundancy | 4.43 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 20-30% PEG 4000, 100mM Na Citrate, 200mM Na Acetate, pH 5.0-6.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
