2QZA
Crystal structure of Salmonella effector protein SopA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97945, 0.97964, 1.06369, 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.732, 68.536, 106.613 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.27061 |
| Rwork | 0.270 |
| R-free | 0.29052 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 35780 | |
| <I/σ(I)> | 27.6 | 3 |
| Completeness [%] | 94.6 | 84 |
| Redundancy | 6.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M Sodium Chloride, 0.1M Bis-Tris(pH5.5), 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
