2QZ8

Crystal structure of Mycobacterium tuberculosis Leucine response regulatory protein (LrpA)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2003-06-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.96400, 1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	146.284, 149.900, 62.506
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.090 - 2.160
R-factor	0.2156
Rwork	0.216
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.743
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.410	2.260
High resolution limit [Å]	2.160	2.160
Rmerge		0.052
Number of reflections	67785
<I/σ(I)>	30.1	16.7
Completeness [%]	99.0	89.8
Redundancy	1.88	1.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291.5	70mM Sodium acetate, 20% Glycerol, 5.6% PEG 4000, pH 6.0, VAPOR DIFFUSION, temperature 291.5K