2QZ7
The crystal structure of a homologue of telluride resistance protein (TerD), SCO6318 from Streptomyces coelicolor A3(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 67.821, 67.821, 170.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.180 - 2.100 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.22546 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.180 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.591 |
| Number of reflections | 24107 | |
| <I/σ(I)> | 45.75 | 4.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.4 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 289 | 0.1M Hepes, 3.4M Na Formate, 40% Jeffamine M-600, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
