2QZ6
First crystal structure of a psychrophile class C beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.600, 69.700, 53.900 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 19.120 - 2.260 |
| R-factor | 0.19224 |
| Rwork | 0.190 |
| R-free | 0.24292 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 2BLT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.405 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.038 | 0.061 |
| Number of reflections | 15724 | |
| <I/σ(I)> | 23.94 | 16.62 |
| Completeness [%] | 95.4 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 20% PEG 6K, 0.1M Tris, pH8.0, vapor diffusion, temperature 298K, VAPOR DIFFUSION |
