2QZ5
Crystal Structure of the C-terminal domain of Aida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.707, 55.732, 86.993 |
| Unit cell angles | 90.00, 100.34, 90.00 |
Refinement procedure
| Resolution | 32.170 - 2.600 |
| R-factor | 0.234 |
| Rwork | 0.225 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qzq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 | 0.316 |
| Number of reflections | 10666 | |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 0.1M Hepes 7.5, 25% PEG 3350, 0.2M Li2SO4, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
