2QYP

Orthorhombic Crystal Structure of Human Saposin C Dimer in Open Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-10-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	C 2 2 21
Unit cell lengths	57.008, 88.904, 93.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.500 - 2.450
R-factor	0.232
Rwork	0.229
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.200	2.540
High resolution limit [Å]	2.450	2.450
Number of reflections	8971
<I/σ(I)>	17	2.1
Completeness [%]	99.0	99.8
Redundancy	2.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	20 mM NaAcetate, 200 mM ammonium sulfate, 30% (v/v) pentaerythriol ethoxylate 15/4, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K