2QYJ
Crystal structure of a designed full consensus ankyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 61 |
| Unit cell lengths | 74.480, 74.480, 50.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.550 - 2.050 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mjo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.550 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.337 |
| Number of reflections | 10232 | 721 |
| <I/σ(I)> | 21.44 | 6.2 |
| Completeness [%] | 92.8 | 48.1 |
| Redundancy | 7.68 | 7.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.7M (NH4)2SO4, 0.1M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
