2QYA
Crystal structure of an uncharacterized conserved protein from Methanopyrus kandleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.252, 49.386, 104.211 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.170 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.29100 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.575 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.640 | 2.290 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.109 | 0.573 |
| Number of reflections | 28952 | |
| <I/σ(I)> | 11.5 | 1.7 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 25% PEG 3350, 200mM Lithium sulfate, VAPOR DIFFUSION, temperature 294K |
