2QY7
Crystal structure of human epsinR ENTH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.9816 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.455, 91.122, 52.893 |
| Unit cell angles | 90.00, 107.13, 90.00 |
Refinement procedure
| Resolution | 36.350 - 2.000 |
| R-factor | 0.20628 |
| Rwork | 0.204 |
| R-free | 0.25465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.169 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.112 | 0.389 |
| Number of reflections | 28679 | |
| <I/σ(I)> | 11.6 | 3.5 |
| Completeness [%] | 99.1 | 95.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 30% (w/v) PEG3000, 100 mM CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
