2QY6
Crystal structure of the N-terminal domain of UPF0209 protein yfcK from Escherichia coli O157:H7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.067, 80.468, 234.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26800 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.544 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.188 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.167 | 0.719 |
| Number of reflections | 46444 | |
| <I/σ(I)> | 6.5 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 4M Sodium formate, pH 7.0, VAPOR DIFFUSION, temperature 294K |
