2QY2
Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domainm.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2006-09-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793, 1.1 |
Spacegroup name | P 64 |
Unit cell lengths | 154.462, 154.462, 39.618 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.090 - 2.000 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 36620 | |
<I/σ(I)> | 31.4 | 1.95 |
Completeness [%] | 99.1 | 95.18 |
Redundancy | 4.61 | 2.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 20-30% PEG 4000 (less than 1 mo. old), 0.1M Sodium Citrate, 0.2M Ammonium Acetate, pH 5.0-6.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |