2QY1
pectate lyase A31G/R236F from Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-01 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.575, 53.310, 73.214 |
| Unit cell angles | 71.64, 80.15, 68.96 |
Refinement procedure
| Resolution | 21.200 - 1.900 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.062 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.340 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 46629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 25% PEG 3350, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
