2QX3
Structure of pectate lyase II from Xanthomonas campestris pv. campestris str. ATCC 33913
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-01 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.183, 53.158, 73.057 |
| Unit cell angles | 71.72, 80.03, 69.10 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.16 |
| Rwork | 0.157 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1air |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.182 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.058 | 0.027 | 0.210 |
| Number of reflections | 35612 | ||
| <I/σ(I)> | 20.1 | ||
| Completeness [%] | 83.6 | 98 | 37.2 |
| Redundancy | 1.9 | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.1M Hepes, 25% polyethylene glycol 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
