2QX2
Structure of the C-terminal domain of sex pheromone staph-cAM373 precursor from Staphylococcus aureus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932, 0.97943 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 69.976, 69.976, 145.954 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.990 - 1.900 |
| R-factor | 0.18318 |
| Rwork | 0.181 |
| R-free | 0.21701 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.427 |
| Number of reflections | 29466 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 90.2 | 57 |
| Redundancy | 8.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 15% Ethanol, 0.1M Tris-HCl pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
