2QWY
SAM-II riboswitch bound to S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Collection date | 2007-04-10 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.258, 48.095, 109.617 |
| Unit cell angles | 90.00, 108.25, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.800 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.26300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 26866 | |
| <I/σ(I)> | 31.9 | 5 |
| Completeness [%] | 97.9 | 94.7 |
| Redundancy | 7.8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 640 mM Ammonium Acetate, 10% PEG 1000, 10 mM Barium Chloride, 50 mM Sodium Cacodylate, 8 mM Cobalt Hexaammine Chloride. Micro-seeded., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Cacodylate | ||
| 10 | 1 | 2 | Cobalt Hexaammine Chloride | ||
| 2 | 1 | 1 | Ammonium Acetate | ||
| 3 | 1 | 1 | PEG 1000 | ||
| 4 | 1 | 1 | Barium Chloride | ||
| 5 | 1 | 1 | Cobalt Hexaammine Chloride | ||
| 6 | 1 | 2 | Sodium Cacodylate | ||
| 7 | 1 | 2 | Ammonium Acetate | ||
| 8 | 1 | 2 | PEG 1000 | ||
| 9 | 1 | 2 | Barium Chloride |
