2QWX

Crystal Structure of Quinone Reductase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	56.635, 83.549, 106.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.500
Rwork	0.192
R-free	0.21300
RMSD bond length	0.013
RMSD bond angle	1.608
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.470
Rmerge	0.066
Number of reflections	84189
<I/σ(I)>	29.8
Completeness [%]	97.9
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	NaCl, Ammonium Sulfate, FAD, Melatonin, DTT, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K