2QWN
Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-386aa)R171C and bovine Auxilin (810-910aa)D876C in the ADP*Pi state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-03 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.627, 58.262, 225.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.710 - 2.400 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.094 | 0.040 | 0.582 |
| Number of reflections | 20512 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 97.3 | 93.4 | 99.9 |
| Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 289 | PEG3350, Ammonium Acetate, pH 8.0, microbatch under oil, temperature 289K |
