2QWL
Crystal structure of bovine hsc70 (1-394aa)in the ADP state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-07 |
| Detector | RIGAKU RAXIS HTC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.306, 77.962, 75.320 |
| Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
| Resolution | 35.160 - 1.750 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.296 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.033 | 0.018 | 0.234 |
| Number of reflections | 83895 | ||
| <I/σ(I)> | 26.5 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 3.6 | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 8 | 289 | PEG3350, Calcium Acetate, pH 8.0, microbatch under oil, temperature 289K |
