2QWA
THE X-RAY STRUCTURE OF A DRUG RESISTANT VARIANT R292K OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE
Experimental procedure
Source type | ROTATING ANODE |
Source details | MACSCIENCE M18X |
Temperature [K] | 107 |
Detector technology | IMAGE PLATE |
Collection date | 1995-07-03 |
Detector | RIGAKU RAXIS II |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 181.400, 181.400, 181.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.700 |
R-factor | 0.171 |
Rwork | 0.171 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TERN N9 NEURAMINIDASE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.820 |
Data reduction software | PROTEIN (V4) |
Data scaling software | PROTEIN (V. 4) |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.076 | |
Total number of observations | 156483 * | |
Number of reflections | 51358 | |
Completeness [%] | 87.0 | 46 |
Redundancy | 3.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.6 * | Laver, W.G., (1984) Virology, 137, 314. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium phosphate | 1.7 (M) | |
2 | 1 | drop | protein | 10-15 (mg/ml) | |
3 | 1 | reservoir | potassium phosphate | 1.9 (M) |