2QW6
Crystal structure of the C-terminal domain of an AAA ATPase from Enterococcus faecium DO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.868, 87.016, 87.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.27500 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.549 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.706 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.149 | 0.662 |
| Number of reflections | 22041 | |
| <I/σ(I)> | 16.1 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.3 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Imidazole pH 6.5, 1M Sodium acetate hydrate, VAPOR DIFFUSION, temperature 294K |
