2QVU
Porcine Liver Fructose-1,6-bisphosphatase cocrystallized with Fru-2,6-P2 and Mg2+, I(T)-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-28 |
| Detector | NOIR-1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.173, 165.627, 79.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.150 - 1.500 |
| R-factor | 0.1961 |
| Rwork | 0.196 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1CNQ |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.213 |
| Total number of observations | 20223 | |
| Number of reflections | 118452 | |
| <I/σ(I)> | 21.5 | 4 |
| Completeness [%] | 93.9 | 65.4 |
| Redundancy | 6.07 | 2.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | see citation, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
