2QVT
Structure of Melampsora lini avirulence protein, AvrL567-D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.474, 45.833, 84.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.260 |
| R-factor | 0.19919 |
| Rwork | 0.196 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2opc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.113 | 0.497 |
| Number of reflections | 7619 | |
| <I/σ(I)> | 11.9 | 8.35 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 7.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES, 1.66 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
