2QVJ

Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	293
Detector technology	CCD
Collection date	2006-12-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	166.456, 236.652, 74.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.620 - 2.800
R-factor	0.246
Rwork	0.243
R-free	0.28600
RMSD bond length	0.006
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.620	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.084	0.172
Number of reflections	54826
<I/σ(I)>	19
Completeness [%]	74.3	44.9
Redundancy	4.62	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K