2QVJ
Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2006-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 166.456, 236.652, 74.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.620 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.28600 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.953 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.620 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 | 0.172 |
| Number of reflections | 54826 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 74.3 | 44.9 |
| Redundancy | 4.62 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K |
