2QVA
Crystal structure of Drosophila melanogaster Translin protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 98.580, 96.620, 153.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.400 |
| R-factor | 0.207 |
| Rwork | 0.203 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qrx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.583 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.099 | 0.354 |
| Number of reflections | 18817 | |
| <I/σ(I)> | 11.8 | 2.6 |
| Completeness [%] | 91.4 | 77.3 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 283 | 10% PEG 4000, 10% isopropanol, 5% glycerol, 100mM sodium citrate buffer, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
