2QV5
Crystal structure of uncharacterized protein ATU2773 from Agrobacterium tumefaciens C58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.349, 47.373, 65.492 |
| Unit cell angles | 91.06, 92.36, 73.06 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.17978 |
| Rwork | 0.178 |
| R-free | 0.23838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nly |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.114 | 0.750 |
| Number of reflections | 33092 | |
| <I/σ(I)> | 4.2 | 0.9 |
| Completeness [%] | 97.7 | 96.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 100mM Sodium cacodylate pH 6.5, 400mM Sodium acetate pH 4.6, 30% PEG8000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
